Seeing what the body says.

A living, intelligent system.

Interstitial fluid surrounds every cell in the body. It carries critical information — including biomarkers tied to inflammation, immune function, and metabolic activity. It’s the biological layer where changes begin.

Learn more about ISF.

Until now, accessing ISF has required invasive procedures or unreliable methods.

KIFFIK changes that with a patented, point-of-care technology that extracts ISF safely, gently, and without pain.

How it works.

It's fast.

It's easy to use.

It's non-invasive.

Our proprietary device uses a brief, controlled electrical pulse — a method known as electroporation — to create temporary microchannels in the outer skin. This opens a  pathway to interstitial fluid, without needles or blood. 

Electroporation

Extraction

How the KIFFIK Lab works.

From micro-pulse to biomarker access —
see how KIFFIK’s technology works.
“The KIFFIK device is unique in generating high-quality ISF samples. The generated samples can be extremely valuable for clinical applications such as faster, easier recruitment of clinical trials, biomarker research and development, assessment of disease mechanisms, for new drug targets, drug repurposing and new combination therapies.”
Ronny Schmidt, PhD
VP Contract Research, Sciomics, GmbH

A deeper dive.

Want to learn more?

interstitial fluid

The following peer-reviewed references highlight the clinical and scientific foundation for using interstitial fluid (ISF) as a biomarker-rich biofluid for diagnostics, wearable monitoring, and future applications in precision health.

  • "Interstitial fluid offers a stable and accessible matrix that mirrors local tissue biochemistry, making it particularly well-suited for monitoring disease activity and therapeutic response in real time."

    Shippy SA, Ofoli R, Schultz M, et al. Interstitial fluid as a source of biomarkers for monitoring tissue status and disease progression. Trends Biotechnol. 2021;39(4):314-325. doi:10.1016/j.tibtech.2020.10.007

  • "ISF contains many of the same analytes as blood, but with less complexity, fewer interferences, and easier access for continuous monitoring using wearable technologies." 

    Heikenfeld J, Jajack A, Rogers J, et al. Wearable sensors: modalities, challenges, and prospects. Lab Chip. 2019;19(6):989-1010. doi:10.1039/C8LC00923A

  • "Recent advancements in ISF extraction techniques have opened the door to continuous, needle-free monitoring of health-related biomarkers, enabling new diagnostic and therapeutic use cases." 

    Mathur S, Sakthivel T, Singh H, et al. Advances in interstitial fluid extraction and analysis for wearable biomarker sensing. Biosens Bioelectron. 2022;196:113732. doi:10.1016/j.bios.2021.113732

  • "Glucose concentrations in interstitial fluid closely mirror blood glucose levels, validating ISF as a reliable substrate for continuous biosensing in diabetes care."

    Hauke A, Nassehi D, Reinemann L, et al. Continuous glucose monitoring in interstitial fluid using minimally invasive microneedle sensor. Biomed Microdevices. 2018;20(4):105. doi:10.1007/s10544-018-0309-5

  • "Although less explored, ISF presents a promising matrix for non-invasive metabolic profiling due to its rich composition and proximity to tissue metabolic activity." 

    Mena-Bravo A, Luque de Castro MD. Sweat: a sample with limited present applications and promising future in metabolomics. J Pharm Biomed Anal. 2014;90:139-147. doi:10.1016/j.jpba.2014.03.009

  • "ISF-based sensors offer unique advantages for transdermal diagnostics, including minimal invasiveness, continuous data capture, and molecular access unavailable through other biofluids." 

    Reddy B, Zuñiga JM, Horowitz L, et al. Transdermal biosensing: technologies and translational challenges. Biosens Bioelectron. 2022;213:114015. doi:10.1016/j.bios.2022.114015

  • "Interstitial fluid extraction provides a viable, less invasive route for real-time biochemical analysis, with applications extending well beyond glucose to hormones, metabolites, and inflammatory markers." 

    Li Y, Liu Y, Guo Z, et al. Noninvasive interstitial fluid extraction for wearable continuous glucose monitoring: advances and challenges. Biosensors. 2021;11(7):195. doi:10.3390/bios11070195

ELECTROPORATION

Electroporation has emerged as a promising method for non-invasive access to interstitial fluid (ISF), enabling wearable diagnostics and continuous biomarker monitoring. The following references provide foundational scientific and clinical insights into the use of electroporation to enhance ISF sampling.

  • "Electroporation provides a minimally invasive method to access ISF by temporarily increasing skin permeability without causing damage or inflammation."
    Zhou J, Liu J, Wang C, et al. Electroporation-based extraction of interstitial fluid for non-invasive glucose monitoring. Biosens Bioelectron. 2018;101:246-252. doi:10.1016/j.bios.2017.10.015
  • "By applying low-voltage pulses, electroporation facilitates rapid and repeatable extraction of ISF suitable for biosensing applications."
    Zhang Y, Liu Y, Wang X, et al. Transdermal interstitial fluid extraction by electroporation for glucose monitoring using a wearable biosensor. J Biomed Nanotechnol. 2020;16(8):1171-1177. doi:10.1166/jbn.2020.2964
  • "Electroporation offers a tunable and reversible increase in skin permeability, creating new opportunities for diagnostic sampling without invasive procedures."
    Apostolou T, Loukas YL. Microporation and electroporation for transdermal drug delivery and diagnostics. Expert Opin Drug Deliv. 2015;12(2):253-262. doi:10.1517/17425247.2015.965690
  • "Although reverse iontophoresis and microneedles have been more widely studied, electroporation remains a promising adjunct or alternative for efficient ISF access."
    Wang PM, Cornwell M, Prausnitz MR. Minimally invasive extraction of dermal interstitial fluid using microneedles and reverse iontophoresis. Pharm Res. 2005;22(7):1201-1209. doi:10.1007/s11095-005-5260-7
  • "Combining microneedle platforms with electroporation may enhance transdermal permeability and ISF collection without compromising skin integrity."
    Park JH, Allen MG, Prausnitz MR. Biodegradable polymer microneedles: Fabrication, mechanics and transdermal drug delivery. J Control Release. 2005;104(1):51-66. doi:10.1016/j.jconrel.2005.02.002
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